Predicting The Impact Of Wood Fibers Processing Using Fluorescent Carbohydrate Binding Modules, 2017PEERS

Wood fiber is a source of raw materials for established industries and for the nascent biofuel sector. Efficient processing of wood fiber polymers such as cellulose requires close monitoring with methods such as FTIR, XPS or chemical analysis. Such methods are time consuming and require the availability of specialized equipment and expertise. Recently, the carbohydrate recognition domains of glycohydrolases, known as carbohydrate binding modules, were shown to be useful in studying the development and the biochemistry of plant cell walls. In this study, we engineered a series of color-coded fluorescent carbohydrate modules with specificities toward four major carbohydrate fiber polymers. This approach allowed for quick and high-throughput analysis of fiber surface carbohydrates signatures. It is hereby used for monitoring and predicting the impact of mechanical and enzymatic carbohydrates signatures. It is hereby used for monitoring and predicting the impact of mechanical and enzymatic carbohydrates signatures. It is hereby used for monitoring and predicting the impact of mechanical and enzymatic 

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