Agrobacterium-mediated Transformation of Hybrid Larch and Production of Transgenic Plants, 1997 Biological Sciences Symposium Proceedings

G. Pilate, S. Dauvillier, V. Levee*, J.-C. Leple, M.-L. Gouez, and

M.-A. Lelu

Station d’Amelioration des Arbres Forestiers

Agrobacteriumn tcmefaciens mediated gene transformation and whole plant regeneration from transformed tissue were established for a conifer species, the hybrid larch Larix kaelqferi x L. clecidun [ 11. A nptll gene under the control of the tandem repeat of the 3% CaMV promoter allowed us to regularly obtain transgenic masses. Using somatic embryogenesis, we are able to easily regenerate numerous plantlets from each of these embryogenic masses (EM). Southern hybridization, using the nptII coding sequence as a probe, were performed on needles collected from greenhouse-grown plants. 14 different plants, each originating from independant kanamycin resistant-EM, have been analysed and all of them appeared to be positive.

Author: Pilate, G., Dauvillier, S., Levee, V., Leple, J.-C., Gouez, M.-L., Lelu, M.-A.
Agrobacterium-mediated Transformation of Hybrid Larch and Pr
Agrobacterium-mediated Transformation of Hybrid Larch and Production of Transgenic Plants, 1997 Biological Sciences Symposium Proceedings
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