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Somatic Embryogenesis has great potential for clonal production of conifer embryos to meet the increased demands of the Pulp and Paper Industry. The quality of embryos, however needs improvement. The similarities and differences between somatic and zygotic embryos are unclear and, to date, gene expression studies have focused on a few proteins, expressed late in development. We have used the technique of Differential Display to examine gene expression throughout the development of somatic and zygotic embryos. Many similarities and differences in gene expression were uncovered. Seventy seven somatic and over one hundred zygotic cDNA ‘bands’ have been cloned and their sequences are being determined. This technique is rapid, simple and sensitive and can provide insights into the physiology of conifer embryogenesis.
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Differential Display: A Tool to Follow Natural and Somatic Embryo Development in Loblolly Pine, 1997